The Brazil international suffered an injury in Wednesday’s remarkable Champions League comeback against Paris Saint-Germain after scoring two goals in the final seven minutes of the 6-1 win that brought a 6-5 aggregate victory.
The Barca players were given Friday off by coach Luis Enrique, but Neymar was unable to take part when they returned to training on Saturday and the decision has been taken not to risk him against Depor.
Rafinha will also miss the game after coming down with a bout of gastroenteritis on Saturday.
The two Brazilians join Aleix Vidal and Jeremy Mathieu on the sidelines, with Luis Enrique calling up Barca B’s Carles Alena to complete his 18-man squad.
Barca begin the weekend a point clear of Real Madrid — who have a game in hand — before Zinedine Zidane’s side host Real Betis later in the day.
And Luis Enrique has called on them to keep their focus after the euphoria of Wendesday night’s extraordinary result.
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